Phylogenic tree of peptide targeting candidates eluted after non-fat milk (NFM) or no blocking of matrix sonicated samples. Peptide sequences were obtained after the third round of screening using the IT9 (PHD 12) protocol, with NFM used to block the non-specific binding to sonicated matrix. All 30 clones analyzed in the NFM-free assay corresponded to a single peptide of 12 amino acids: AHKHKPGHITA. Structural relatedness using MacVector sequence analysis software. Recovery of increased 800-fold in IT9 biopanning.
Sawada et al. Fibrogenesis & Tissue Repair 2011 4:7 doi:10.1186/1755-1536-4-7