Human PBMCs cultured in the presence of TLR2 agonists are viable. Human PBMCs were cultured in the (A). absence or (B) presence of 1.79 μg/ml TLR2 agonist LTA or (C) 0.89 μg/ml TLR7 agonist IMIQ. After 5 days, the cells cultured in different conditions were photographed. Bar is 20 μm. (D) Human PBMCs were cultured in the absence (SFM control) or presence of 0.89 μg/ml TLR2 agonists Pam3CSK4, 8.9 × 107 cells/ml HKLM, 0.89 μg/ml LM-MS, 1.79 μg/ml LTA, 8.9 μg/ml PG-LPS or 0.89 μg/ml FSL-1 for 5 days. After 5 days, the cells were photographed and the number of cells per picture was counted. The results are means ± SEM of cells per 0.15 mm2 (n = 3 separate experiments).
Maharjan et al. Fibrogenesis & Tissue Repair 2010 3:23 doi:10.1186/1755-1536-3-23