Figure 8.

Serum amyloid P (SAP) has no effect on fibroblast proliferation or transforming growth factor (TGF)-β-mediated epithelial to mesenchymal transition (EMT). (A) Normal human dermal fibroblasts (NHDF) were stimulated with platelet-derived growth factor (PDGF; 200 ng/mL) for 24 hours in the presence or absence of SAP. Proliferation was quantified using 5-bromo-2-deoxyuridine incorporation. (B) A549 cells were stimulated with TGF-β1 in the presence or absence of SAP for 48 hours. The resultant lysates were assessed for the epithelial marker E-cadherin or the mesenchymal marker extra domain-A (EDA)-fibronectin using western blotting analysis. *P < 0.05, **P < 0.01, ***P < 0.005.